Recently, I have developed a tool named MetaLogo, aimed to make sequence logos for multiple sets of sequences with different lengths or other characteristics.
We all know WebLogo could make sequence logos, however, together with other sequence logo generators, these tools can only accept sequences with equal lengths. Although a multiple sequence alignment (MSA) seems to be a solution, but that’s not perfect at all. We just sometimes need to discriminate the length of sequences. For example ,when we analyze immune repertoire, we definitely know that a 14-bp CDR3 and a 30-bp CDR3 could totally target to different antigens and a MSA helps nothing. We need to divide the sequences into different groups by their lengths. Then we need a tool to draw sequence logos for each group and put them together in one figure. If the tool could make some alignment and padding for these sequence logos to highlight the similarities or differences among different group of sequences, that would be perfect!
Therefore, we developed MetaLogo!
You can directly input your sequence file with thousands of sequences of different lengths and make your aligned multiple sequence logos. You can also group your sequences by other criterion besides lengths.
What’s more, MetaLogo could make beautiful and highly customizable sequence logos. You can visit the public web server of it (http://metalogo.omicsnet.org), or you can install the python package (pip install MetaLogo). You can even easily build your own web server in your local network. A temp development server and a efficient production docker server are both available.
That’s amazing. Just try it. Here is the tutorial: https://github.com/labomics/MetaLogo/wiki and here is the webserver (http://metalogo.omicsnet.org).
If you have any question, please leave a message here, or create a issue on github, you can also send me emails (find address in the MetaLogo webserver).
2018 年注定是不平凡的一年，越来越多的区块链项目出现在视野中，越来越多的超级学术大牛也成为了各种项目的创始人、合作者、顾问。值此百家争鸣之际，我们也抽空来膜拜一下数字货币诞生初期的众多神秘的大牛们。今天我们八卦一下 Wei Dai 大佬。
Wei Dai 是谁？如果读一下比特币白皮书，会发现其中第一篇参考文献就是来自于 Wei Dai 的 b-money。大部分对比特币起源的报道文章也都会提到 b-money。b-money究竟是什么？我们引用一下以太坊白皮书里的介绍：
In 1998, Wei Dai’s b-money became the first proposal to introduce the idea of creating money through solving computational puzzles as well as decentralized consensus, but the proposal was scant on details as to how decentralized consensus could actually be implemented.
什么意思呢？翻译一下就是说，b-money 第一个提出通过解决计算机难题和达成去中心化的共识来产生电子货币。在1998年，这个理念相当创新和超前，在当时的环境下这样的系统只能部署在脑海，所以 b-money 中并未提及具体的一些共识实施细节。但这恰恰说明了 Wei Dai 的犀利之处，之后的 Hal Finney 和 Adam Back 等人的想法都与 Wei Dai 的 b-money 有关。